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【求助】大神帮忙翻译和解释这一小段话
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Data were analyzed using the statistical software package, SAS9.1 (SAS Institute, Cary, NC). The levels of circulating exosomes for each group of patients were defined as means ± standard deviations from at least two separate experiments performed in triplicate. Comparisons between these groups were performed by one-way ANOVA, followed by the Tukey's multiple comparisons post-test comparing each population. Relative quantification of microRNAexpression was calculated with the 2?ΔΔCt method (Applied Biosystems User Bulletin No. 2) and data were analyzed as log10 of relative quantity (RQ) of the target microRNA, normalized with respect to control microRNA added to each sample, allowing comparisons between arrays. The microRNA distributions and correlations along with confidence intervals were calculated for each subset. Statistical significance was set asp≤0.05.
哪位大神帮忙翻译一下这一小段。能就里面统计和实验的东西做一些解释就更好了。
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lifepassenger Data were analyzed using the statistical software package, SAS9.1 (SAS Institute, Cary, NC). The levels of circulating exosomes for each group of patients were defined as means ± standard deviations from at least two separate experiments performed in triplicate. Comparisons between these groups were performed by one-way ANOVA, followed by the Tukey's multiple comparisons post-test comparing each population. Relative quantification of microRNAexpression was calculated with the 2?ΔΔCt method (Applied Biosystems User Bulletin No. 2) and data were analyzed as log10 of relative quantity (RQ) of the target microRNA, normalized with respect to control microRNA added to each sample, allowing comparisons between arrays. The microRNA distributions and correlations along with confidence intervals were calculated for each subset. Statistical significance was set asp≤0.05.
哪位大神帮忙翻译一下这一小段。能就里面统计和实验的东西做一些解释就更好了。 使用SAS9.1进行统计数据分析。每个组患者的循环外泌体（CE）以均数±标准差的形式给出，数据来自至少重复三次的两组独立实验。不同组间的比较采用单因素方差分析，用Tukey多重比较检验进行两两比较。microRNA相对定量使用2-ΔΔCt方法计算，目标microRNA的相对定量取log10进行转化，并参照加入各样本的对照microRNA进行标化，然后进行分析，这样能够让不同批次的测量可比。计算每个亚组microRNA的置信区间来描述分布和相关性使用。显著性取0.05（asp不知道是什么？原文就是这样？）总体意思就是，测试的数据要得先取对数，然后标化，然后用ANOVA，然后用tukey两两比较，然后计算置信区间
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谢谢回复！！
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Tumor exosomes were isolated from the first aliquot by the MACS procedure immediately and total RNA was isolated and stored at ?70 °C until isolation of all samples. The remaining sera samples were stored at 4 °C for subsequent exosome isolation. Tumor exosomes were isolated from the second aliquot after 24 h, from the third aliquot after 48 h and from the fourth sample after 96 h at 4 °C. RNA was isolated from each exosome preparation and stored. In a similar study, 3 additional serum aliquots were stored at ?70 °C for 7 to 28 days, prior to exosome and RNA isolations to mimic the use of banked specimens.这是第四段。
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师哥好，拜读了您给我在求助里的翻译，佩服的五体投地。这是那一段前面的一小段，是一个实验的方法学，希望您百忙之中能不辞辛苦帮忙翻译一下，小弟万分感谢。丁当我给您转帐。Isolation and profiling of microRNATotalRNAwas isolated from the tumor cells and exosomes using the mirVana microRNA isolation kit according to manufacturer's instructions (Ambion, Austin, TX). The RNA quality, yield, and size of microRNA fractions were analyzed using Agilent 2100 Bioanalyzer (Agilent Technologies, Foster City, CA). The isolated microRNAs were 3′-end labeled with Cy3 using the mirVana microRNA Array Labeling Kit (Ambion) and the Post Labeling Reactive Dye kit (Amersham Bioscience, Pittsburgh, PA). MicroRNA profiling was performed in duplicate by Ocean Ridge Biosciences (Jupiter, FL) using microarrays containing probes for 467 human mature microRNAs. This analysis used custom-developed microRNA arrays covering the 467 microRNAs present in the Sanger Insitute mirBASE v9.0, consisting of 35–44-mer oligonucleotides, manufactured by Invitrogen and spotted in duplicate. After hybridization, the microRNA arrays were scanned using a GenePix 4000A array scanner (Axon Instruments, Union City, CA) and the raw data normalized and analyzed using GeneSpring 7.0Software (Silicon Genetics, Redwood City, CA). Normalization was performed by expressing each microRNA replicate relative to control microRNA (Ambion) added to each sample, allowing comparisons between arrays. Threshold and 95th percentile of negative controls (TPT95) were calculated based on hybridization signal from negative control probes including: 38 mismatch and shuffled control probes and 87 non-conserved Caenorhabditis elegans probes. To define sensitivity, NCode synthetic microRNA was spiked at 1/500,000 mass ratio into labeling reactions and the signal intensity was detected. For specificity, perfect match probes for miR-93, miR-27a, and miR-152 and 2 mismatches for each were used. The 2 base pair mismatch probes demonstrated a signal below or at TPT95 on all arrays. To assess the stability of the exosomal profiling with storage and manipulations, sera from patients with ovarian cancer patients were obtained and aliquoted into four 4 ml samples. Tumor exosomes were isolated from the first aliquot by the MACS procedure immediately and total RNA was isolated and stored at ?70 °C until isolation of all samples. The remaining sera samples were stored at 4 °C for subsequent exosome isolation. Tumor exosomes were isolated from the second aliquot after 24 h, from the third aliquot after 48 h and from the fourth sample after 96 h at 4 °C. RNA was isolated from each exosome preparation and stored. In a similar study, 3 additional serum aliquots were stored at ?70 °C for 7 to 28 days, prior to exosome and RNA isolations to mimic the use of banked specimens.师哥，我直接发到这里吧
您看着舒服点。另外，我邮箱，您可以给我发邮件，多谢师哥！！
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lifepassenger 师哥好，拜读了您给我在求助里的翻译，佩服的五体投地。这是那一段前面的一小段，是一个实验的方法学，希望您百忙之中能不辞辛苦帮忙翻译一下，小弟万分感谢。丁当我给您转帐。Isolation and profiling of microRNATotalRNAwas isolated from the tumor cells and exosomes using the mirVana microRNA isolation kit according to manufacturer's instructions (Ambion, Austin, TX). The RNA quality, yield, and size of microRNA fractions were analyzed using Agilent 2100 Bioanalyzer (Agilent Technologies, Foster City, CA). The isolated microRNAs were 3′-end labeled with Cy3 using the mirVana microRNA Array Labeling Kit (Ambion) and the Post Labeling Reactive Dye kit (Amersham Bioscience, Pittsburgh, PA). MicroRNA profiling was performed in duplicate by Ocean Ridge Biosciences (Jupiter, FL) using microarrays containing probes for 467 human mature microRNAs. This analysis used custom-developed microRNA arrays covering the 467 microRNAs present in the Sanger Insitute mirBASE v9.0, consisting of 35–44-mer oligonucleotides, manufactured by Invitrogen and spotted in duplicate. After hybridization, the microRNA arrays were scanned using a GenePix 4000A array scanner (Axon Instruments, Union City, CA) and the raw data normalized and analyzed using GeneSpring 7.0Software (Silicon Genetics, Redwood City, CA). Normalization was performed by expressing each microRNA replicate relative to control microRNA (Ambion) added to each sample, allowing comparisons between arrays. Threshold and 95th percentile of negative controls (TPT95) were calculated based on hybridization signal from negative control probes including: 38 mismatch and shuffled control probes and 87 non-conserved Caenorhabditis elegans probes. To define sensitivity, NCode synthetic microRNA was spiked at 1/500,000 mass ratio into labeling reactions and the signal intensity was detected. For specificity, perfect match probes for miR-93, miR-27a, and miR-152 and 2 mismatches for each were used. The 2 base pair mismatch probes demonstrated a signal below or at TPT95 on all arrays. To assess the stability of the exosomal profiling with storage and manipulations, sera from patients with ovarian cancer patients were obtained and aliquoted into four 4 ml samples. Tumor exosomes were isolated from the first aliquot by the MACS procedure immediately and total RNA was isolated and stored at ?70 °C until isolation of all samples. The remaining sera samples were stored at 4 °C for subsequent exosome isolation. Tumor exosomes were isolated from the second aliquot after 24 h, from the third aliquot after 48 h and from the fourth sample after 96 h at 4 °C. RNA was isolated from each exosome preparation and stored. In a similar study, 3 additional serum aliquots were stored at ?70 °C for 7 to 28 days, prior to exosome and RNA isolations to mimic the use of banked specimens.师哥，我直接发到这里吧 您看着舒服点。另外，我邮箱，您可以给我发邮件，多谢师哥！！好的 下次发帖记得@ 一下哈
不然看不见 …
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师哥，邮件收到了。翻译的真好，谢谢师哥。叮当怎么给您？
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@veraxu，谢谢师哥，最近没上丁香园。叮当给晚了，还请见谅！！
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veraxu @lifepassenger：Data were analyzed using the statistical soft...使用SAS9.1进行统计数据分析。每个组患者的循环外泌体（CE）以均数±标准差的形式给出，数据来自至少重复三次的两组独立实验。不同组间的比较采用单因素方差分析，用Tukey多重比较检验进行两两比较。microRNA相对定量使用2-ΔΔCt方法计算，目标microRNA的相对定量取log10进行转化，并参照加入各样本的对照microRNA进行标化，然后进行分析，这样能够让不同批次的测量可比。计算每个亚组microRNA的置信区间来描述分布和相关性使用。显著性取0.05（asp不知道是什么？原文就是这样？）总体意思就是，测试的数据要得先取对数，然后标化，然后用ANOVA，然后用tukey两两比较，然后计算置信区间
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跪求大神帮忙翻译一下简历
个人履历：2008年11月份进入xx公司，08年11月到10年7月在生产线kitting工作期间去培训组装电脑，2010年7月在生产线参加考试进入ARB做EMR,在岗期间主要负责维修台式机和笔记本，另外负责日本、韩国退回中国的机器维修、测试，和对运营商的一些交接，日常工作现场的安排.
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有个投稿要求看不懂！！求大神们帮忙翻译！！
The final figure sizes are: 1 column = 3-in. (8.25-cm) wide, 1.5 column = 5-in. (13-cm) wide, 2 columns = 6-in. (17.15-cm) wide. Figures should not exceed 8-in. (21.6-cm) in height. All cropping and manipulation must be completed before the images are submitted to the publisher.
上述的是啥意思啊。。求解答
/journal/10.1002/(ISSN)/homepage/ForAuthors.html
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