Transwell Assay
Transwell (Boyden Chamber) Cell
Migration Assay
Quan Kang, 6/6/2004; commented
by T.-C. He
Reagents and Materials
1.Transwell Unit:
Corning-Costar 3494 Transwell. 燩TFE membrane and polystyrene plate, 12 wells
plate, 3.0祄 pore size membrane, pre-coated with collagen types I and IV. (Fisher Cat # 07-200-561).
2.BSA media: DMEM,
0.1%BSA (Note: you can add P/S to the
media for long-term storage).
3.Q-tips (cotton
swabs), 10%formalin, Hematoxylin, Permont
Transwell (Boyden
Chamber) Assay Using Soluble Chemoattractents
1.Pre-warm BSA
media and Complete media at 37oC
2.Prepare desired
concentration of chemoattractant solution with BSA
media (without FBS), for 12 wells
plate, 1ml/well will be enough.
migratory cells (e.g., in T-25): Trypsinize with 1ml Trypsin and resuspend cell in suspension
with 5ml complete media.}