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&&&&This peptide comprises three fragments, an arginime-glycine-aspartic acid tripeptide motif to recognize the integrin receptor in the cell membrane, a nuclear localization signal sequence to recognize the nuclear pore complexes in nuclear envelope and a short stretch of 12 lysine residues to enhance the peptide binding to DNA.
&&&&该肽载体由能识别细胞膜上整合素（integrin）受体的RGD（Arg-Gly-Asp）三肽基序、识别核膜上核孔复合体（nuclear porecomplexes，NPC）的核内化信号（nuclear localization signal，NLS）序列以及可增加与DNA结合的12个赖氨酸残基三个部分组成。
&&&&We suggested and prepared immunogens by condensation of LEK or MEK with poly-Llysine semisuccinic acid, the rate of conjugation was 81% or 90%, one LEK or MEK molecule per 3.2 or 2.6 lysine residues.
&&&&作者提议并合成了多聚赖氨酸琥珀酰脑啡肽免疫原,EK结合率81～90%,每2.3或3.2个赖氨酸残基连上一个EK。
&&&&EFFECT ON THE INFECTION AND RECONSTITUTION OF HRV_(sh) BY THE MODIFICATION OF LYSINE RESIDUES
&&&&长叶车前花叶病毒上海分离株外壳蛋白赖氨酸残基的修饰对感染及重组的影响
&&&&The reversible acetylation/deacetylationmodification of lysine residues in core histone tails mediated by these two enzymes modulatesthe initiation of transcription, thus leads to the activation/suppression of gene expression.
&&&&这两种酶通过对核心组蛋白尾部赖氨酸残基进行可逆的乙酰化修饰来调控转录的起始,并由此介导基因的激活或抑制。
&&&&As a transcription elongation factor, it plays important roles in recruiting chromatin remodeling proteins, and in modulating chromatin structure by acetylating the highly conserved lysine residues in N-terminal tails of histones.
&&&&通过乙酰化特异染色质区组蛋白尾部的赖氨酸残基,Elp3参与特异染色质区结构的改变,为染色质结构调控蛋白提供识别信号,在转录延伸中发挥重要的作用。
&&&&This peptide comprises three fragments, an arginime-glycine-aspartic acid tripeptide motif to recognize the integrin receptor in the cell membrane, a nuclear localization signal sequence to recognize the nuclear pore complexes in nuclear envelope and a short stretch of 12 lysine residues to enhance the peptide binding to DNA.
&&&&该肽载体由能识别细胞膜上整合素（integrin）受体的RGD（Arg-Gly-Asp）三肽基序、识别核膜上核孔复合体（nuclear porecomplexes，NPC）的核内化信号（nuclear localization signal，NLS）序列以及可增加与DNA结合的12个赖氨酸残基三个部分组成。
&&&&We suggested and prepared immunogens by condensation of LEK or MEK with poly-Llysine semisuccinic acid, the rate of conjugation was 81% or 90%, one LEK or MEK molecule per 3.2 or 2.6 lysine residues.
&&&&作者提议并合成了多聚赖氨酸琥珀酰脑啡肽免疫原,EK结合率81～90%,每2.3或3.2个赖氨酸残基连上一个EK。
&&&&There are two lysine residues in the HRVsh coat protein subunit as re-ported in a previous paper.
&&&&长叶车前花叶病毒上海分离株外壳蛋白中有二个赖氨酸残基。
&&&&If the reaction was carried out in the presence of 4M urea,two lysine residues and one cysteine sulfhydryl group were simultanouslyblocked.
&&&&如果有4M尿素存在,则有两个赖氨酸残基和一个半胱氨酸巯基被修饰。
&&&&Its C terminal was composed of multiple lysine residues, which played as a DNA condensing agent, whereas the N terminal was the receptor binding domain of Epidermal Growth Factor(N??32?～K??48?).
&&&&方法人工合成了一种N端含多个赖氨酸残基，C端为人表皮生长因子（EGF）的受体结合域的33肽，此合成肽（K9E肽）既具有DNA结合活性，又能为细胞表面EGF受体所识别并内在化。
&&&&The optimum temperature of the trypsin was 40 ℃ using Boc-Leu-Arg-Arg-MCA as substrate and the optimum pH was 9.0.Substrate specificity analysis revealed that the trypsin specifically cleaved at the carboxy1 side of the arginine and lysine residues.
&&&&以Boc-Leu-Arg-Arg-MCA为底物测得该酶的最适温度为40℃,最适pH值为9.0.丝氨酸蛋白酶抑制剂(Pefab loc SC,PMSF,Benzam id ine)对该酶的活力有明显抑制作用.
&&&&SPIN LABELING OF THE SERINE AND LYSINE RESIDUES OF BACTERIORHODOPSIN IN ITS MEMBRANE ENVIRONMENT
&&&&细菌视紫红质蛋白的赖氨酸和丝氨酸残基在其膜环境中的自旋标记研究
&&&&Human Elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone H3 and H4 during transcription elongation in higher cells.
&&&&的HAT活性行使调控功能。 4、利用点突变和Plasmid Shuffle等技术构建了含H3 K14一R、H4 KS~R单突变和H3 K14一侧H4 KS一R双突变的e1P3乙菌株，通过功能互补实验分析少凡P3对突变株的补偿功能，证实酵母Elongator可对H3 14K/H4 SK进行乙酞化修饰。
&&&&These peptides (eg. oligolysine, [K] ~) can bind with plasmid DNA throughelectrostatic interactions between the positively charged lysine residues and thenegatively charged phosphate backbone of the DNA, forming highlycondensed particles that allow internalization by mammalian cells andprotection of the packaged DNA from the effects of nucleases.
&&&&其中，阳离子肽类DNA转运载体为一些富含正电荷氨基酸的多肽，如寡聚赖氨酸中，［K］＊，它们可通过电性中和作用与富含负电荷（磷酸根基团）的质粒DNA发生聚合、压缩，从而将直径约数百纳米、松散的质粒DNA聚缩成为几十纳米的致密颗粒，使之易于被真核细胞摄入并保护DNA免于核酸酶的降解，如此达到基因转染的目的。
&&&&One of the lysine residues located on the surface of the virion of HRVsh was modified by trinitriphenylsulfonic acid under mild condition.
&&&&从三硝基苯磺酸修饰的病毒颗粒(TNP-HRVsh)中分离得到的RNA能与天然的HRVsh的外壳蛋白重建病毒颗粒,并具有感染力,说明修饰过程中核酸并不受影响。
查询“lysine residues”译词为用户自定义的双语例句&&&&我想查看译文中含有：的双语例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。&&&&&&&&&&&& Since leu-enkephalin (LEK) and met-enkephalin (MEK) were discovered in 1975, great progress has been made in the research on brain opiate pentapeptides. The preferable estimation method for these peptides is radioimmunoassay (RIA). We suggested and prepared immunogens by condensation of LEK or MEK with poly-Llysine semisuccinic acid, the rate of conjugation was 81% or 90%, one LEK or MEK molecule per 3.2 or 2.6 lysine residues. Rabbits were immunized by multiple intradermal inoculation. Specific antiserum... Since leu-enkephalin (LEK) and met-enkephalin (MEK) were discovered in 1975, great progress has been made in the research on brain opiate pentapeptides. The preferable estimation method for these peptides is radioimmunoassay (RIA). We suggested and prepared immunogens by condensation of LEK or MEK with poly-Llysine semisuccinic acid, the rate of conjugation was 81% or 90%, one LEK or MEK molecule per 3.2 or 2.6 lysine residues. Rabbits were immunized by multiple intradermal inoculation. Specific antiserum (ALS or AMS) was obtained 6 or 3 months afterwards respectively, the working dilution of which was 1:5000, K_(eff) about 10~9 liters per mole. ~(125)I labelled LEK or MEK with specific activity of about 1,600μ Ci/μg were prepared by a modified chloramine-T method and purified by DEAE-Sephadex A-25 column chromatography, and were shown to possess a high immunoreactivity. The double-antibody technique was used to separate the "bound" from "free" ~(125)I-enkephalins. In the competitive inhibition curves, the detection limit of LEK was～ 11pg and that of MEK～130pg, whereas the precision was 1.8 or 1.4% respectively. No significant interference by β-endorphin, 8 peptide hormones, morphine and naloxone was found. The cross reaction rate was low, i. e., MEK vs. ALS was 3.3% and LEK vs. AMS was less than 1%.Brain enkephalins were extracted with 0.1N HCl. After neutralization, the supernatant portions were used directly for RIA. Several tests for validating the methodology has been processed: non-specific combination was found to bo less than 0.4～1.8%; a linear relation was found between the amounts of authentic MEK added to brain extract and the values estimated (p<0.001); the recovery of authentic LEK added to brain tissue was 84.2±8.2% (C.V.=9.7%, n=6): increasing the volumes of added brain extract increases the estimated quantity of enkephalins proportionally (p<0.001); the intraassay coefficient of variation of brain extracts measured was 3.4～6.2%, whereas the interassay coefficient of variation was 6.0～19.3%. In the normal rat brain, the highest amount of enkephalins, in terms of unit wet weight, was in the hypothalamus, with the caudate the next. These two assays have been shown to be sensitive, reliable and specific in our routine assay.1975年发现亮氨酸脑啡肽(LEK)和甲硫氨酸脑啡肽(MEK)。其测定以放射免疫(RIA)最佳。作者提议并合成了多聚赖氨酸琥珀酰脑啡肽免疫原,EK结合率81～90%,每2.3或3.2个赖氨酸残基连上一个EK。免疫3或6个月时获得抗EK血清(ALS或AMS),工作稀度可达1:5000,K_(eff)10~9升/克分子。试用改进的氯胺T标记法及DEAE-Sephadex A-25柱层析制备高比放射性~(125)I-EK免疫活性良好。双抗体法分离抗体结合的和游离的~(125)I-EK。竞争抑制曲线:灵敏度LEK～11微微克,MEK～130微微克;精密度1.8%及1.4%。β-内啡肽,八种肽类激素、吗啡、纳洛酮无明显干扰;交叉反应MEK对ALS为3.3%、LEK对AMS为<1%。脑组织用0.0N盐酸抽提,中和后,直接作RIA。本方法已通过多种方法学考验。正常大鼠脑中EK浓度以下丘脑为最高,尾核次之。本法是灵敏、可靠和特异的。 1. Snake muscle aldolase was purified by ammonium sulphate fractionation and followed by affinity chromatography. Its molecular weight is about 160,000 daltons by gel filtration and consists of four identical subunits of 40,000 daltons shown by SDS gel electrophoresis. Therefore, snake muscle aldolase belongs to class I type A, and every intact subunit in its tetramer has catalytic activity.2. The effects of methanol or urea on the activities of snake and rabbit aldolases are different. When snake and rabbit... 1. Snake muscle aldolase was purified by ammonium sulphate fractionation and followed by affinity chromatography. Its molecular weight is about 160,000 daltons by gel filtration and consists of four identical subunits of 40,000 daltons shown by SDS gel electrophoresis. Therefore, snake muscle aldolase belongs to class I type A, and every intact subunit in its tetramer has catalytic activity.2. The effects of methanol or urea on the activities of snake and rabbit aldolases are different. When snake and rabbit aldolases were subjected to tryptic digestion respectively, it was found that the snake aldolase is more prone to attack.These results suggest that snake aldolase has a more rigid conformation. The hydrophobic bonds inside the molecules of the enzyme, which were destroyed by high concentrations of organic solvent but not by low concentrations of organic solvent, may play an important role in maintaining the active form of the enzyme and on the other hand the polar groups on the surface of the enzyme, including arginine and lysine residues forming ionic pairs, may make it more easily attacked by trypsin and at the same time may prevent the organic solvent at low concentration from contact with the hydrophobic bonds inside the molecules of the enzyme.3. The reactions between aldolase and TNBS suggest that the amino group or groups, in snake aldolase, may play a special role in maintaining its native conformation.4. There are some conformational differences between snake and rabbit aldolase, but the hybridization can still be performed. The results show that from the two different speoies the conformation of the domains of binding of the subunits of the two aldolases, however, are not significantly different.(1)用硫酸铵分级,然后磷酸纤维素亲和层析得到了均一的蛇肌醛缩酶,凝胶过滤法测定全酶分子量为160,000,SDS-凝胶电泳法测定了它的亚基分子量为40,000,表明蛇肌醛缩酶是一个四亚基的酶,它属于醛缩酶I-A型。(2)甲醇或尿素对蛇肌醛缩酶和兔肌醛缩酶的影响是不同的。当用胰蛋白酶分别消化蛇肌醛缩酶和兔肌醛缩酶时,发现蛇肌醛缩酶对胰蛋白酶的作用更为敏感。甲醇或尿素对酶活性影响的结果说明蛇肌醛缩酶有更紧密的构象,酶分子内部的疏水键在维持活化型时起着重要的作用,在低有机溶剂浓度时不受影响,而在高有机溶剂浓度时分子内部疏水键才遭到破坏。此外蛇肌醛缩酶分子表面的极性基团,包括赖氨酸和精氨酸形成的离子对可能更易受到胰蛋白酶作用,同时它们可阻止低浓度甲醇与酶分子内疏水键作用。(3)醛缩酶和TNBS之间的反应支持酶分子的氨基,尤其是蛇肌醛缩酶的氨基在维护其天然构象中起着特殊作用的观点。(4)虽然蛇肌醛缩酶和兔肌醛缩酶的构象有一定的不同,但是仍然能够进行杂交产生蛇肌-兔肌醛缩酶杂交株组,说明它们亚基之间的结合区域的差别并不很大。 There are two lysine residues in the HRVsh coat protein subunit as re-ported in a previous paper.Either the intact virus particles or the coat pro-tein of HRV_(sh) were incubated with trinitrophenylsulfonic acid at pH 8.5.It was found that only one lysine resi-due was modified with trinitropheny-lsulfonic group.If the reaction was carried out in the presence of 4M urea,two lysine residues and one cysteine sulfhydryl group were simultanouslyblocked.The experiment of infection shows that the... There are two lysine residues in the HRVsh coat protein subunit as re-ported in a previous paper.Either the intact virus particles or the coat pro-tein of HRV_(sh) were incubated with trinitrophenylsulfonic acid at pH 8.5.It was found that only one lysine resi-due was modified with trinitropheny-lsulfonic group.If the reaction was carried out in the presence of 4M urea,two lysine residues and one cysteine sulfhydryl group were simultanouslyblocked.The experiment of infection shows that the TNP-modified HRVsh lost its infectivity over ninety percent.The TNP-protein of HRV_(sh) has lost its ability to undergo reconstitution with RNA.These results clearly in-dicated that one lysine residue is lo-cated on the surface of the virion and another is hidden inside.The former seems to be essential for the infection of HRV_(sh).长叶车前花叶病毒上海分离株外壳蛋白中有二个赖氨酸残基。完整的病毒颗粒或外壳蛋白在pH8.5条件下,只有一个赖氨酸残基与三硝基苯磺酸反应。如果有4M尿素存在,则有两个赖氨酸残基和一个半胱氨酸巯基被修饰。三硝基苯磺酰化的HRV_(sh)病毒颗粒的感染力丧失90%以上。HRV_(sh)的外壳蛋白经三硝基苯磺酸修饰后不能与HRV_(sh)-RNA重建。这些结果清楚地表明两个赖氨酸残基中一个位于病毒颗粒表面,另一个则包在病毒颗粒中。表面那个赖氨酸残基为HRV_(sh)的感染所必需。&nbsp&&&&&相关查询
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中国学术期刊(光盘版)电子杂志社长期服用赖氨酸有什么副作用吗？我家孩子个子小，吃饭不好，听别人说服用赖氨酸能长个子，请问长期服用赖氨酸有什么副作用吗？服用时有没有什么忌口，补钙，补维生素影响吗？
赖氨酸是人体必需的21种氨基酸中的一种。而且是人体内无法通过氨基转换得到的一种氨基酸。简单地说，这种氨基酸人体必需，但人体内又无法自身合成。所以它的来源必须是通过饮食获得。
有研究证实，赖氨酸与大脑的发育与神经的营养有关。
但从赖氨酸的来源来看，其来源很广泛。一般的动物食品和豆类都有相当丰富的赖氨酸。所以保证正常的饮食，不偏食，是不会缺的。分过来，如果饮食不正常，那什么都可能缺。所以应当纠正饮食问题。
赖氨酸在上世纪80年代被吹的神乎其神，夸大了。
适当补充没有什么副作用，但把它当作灵丹妙药，则误入其途。
其他答案（共3个回答）
家称它为人体第一必需氨基酸。
近年来，科学家还发现...
氨酸就是人体需要的一种氨基酸，一种不可缺少的营养物质。蛋白质是构成人体细胞的主要成份，食物中的蛋白质进入人体后经过消化先分解成氨基酸，然后人体又利用这些氨基酸再合成新的人体蛋白质，如免疫抗体、消化酶、血浆蛋白、生长激素等都是合成后的人体蛋白质。
在合成蛋白质的各种氨基酸中，赖氨酸是最重要的一种，少了它，其它氨基酸就受到限制或得不到利用，相关信息家称它为人体第一必需氨基酸。
近年来，科学家还发现，赖氨酸是控制人体生长的重要物质抑长素 (Somatotation,ss) 中最重要的也是最必需的成份，对人的中枢神经和周围神经系统都起着重要作用。人体不能自身合成赖氨酸，必须从食物中吸取。每天服用可以促进身体生长,孕妇及哺乳期妇女用药,肾功能减退者,应酌情减量,或遵医嘱,服用无忌口,可与补钙,补维生素同时进行
赖氨酸是人体必需的21种氨基酸中的一种。而且是人体内无法通过氨基转换得到的一种氨基酸。简单地说，这种氨基酸人体必需，但人体内又无法自身合成。所以它的来源必须是通...
亲爱的 建议您带孩子就医给孩子做一些化验在听医嘱
赖氨酸为碱性必需氨基酸。由于谷物食品中的赖氨酸含量甚低，且在加工过程中易被破坏而缺乏，故称为第一限制性氨基酸。赖氨酸可以调节人体代谢平衡。赖氨酸为合成肉碱提供结...
您好，很高兴为您解答，一般在近百元左右，宝宝一次半包，一天两次就可以了，必须注意合理的休息和必要饮食的调理。祝您健康
病情分析：你好，赖氨酸具有促进人体生长发育的作用；肌醇能促进肝中脂肪代谢；维生素B12是体内合成DNA的重要辅酶。指导意见：吃了这个药食欲恢复，精神状态也恢复说...
维生素是生物的生长和代谢所必需的微量有机物。分为脂溶性维生素和水溶性维生素两类。前者包括维生素A、维生素D、维生素E、维生素K等，后者有B族维生素和维生素C。人...
维生素是生物的生长和代谢所必需的微量有机物。分为脂溶性维生素和水溶性维生素两类。前者包括维生素A、维生素D、维生素E、维生素K等，后者有B族维生素和维生素C。人...
维生素是生物的生长和代谢所必需的微量有机物。分为脂溶性维生素和水溶性维生素两类。前者包括维生素A、维生素D、维生素E、维生素K等，后者有B族维生素和维生素C。人...
维生素是生物的生长和代谢所必需的微量有机物。分为脂溶性维生素和水溶性维生素两类。前者包括维生素A、维生素D、维生素E、维生素K等，后者有B族维生素和维生素C。人...
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DNA电极修饰技术及其在
环境电化学中的应用研究
脱氧核糖核酸（DNA）是生命体的基因物质，在生物的生命过程中起着十分重要的作用。DNA承担着生命体的信息遗传，通过自我复制和表达来构建细胞内的生物蛋白和酶的生物合成。对DNA的分析在生物化学和分子生物学研究中有着极为重要的意义。自1953年Watson和Crick发现生物遗传分子脱氧核糖核酸的双螺旋结构以后，人们对于DNA的研究给予越来越多的关注，尤其是对于DNA与其他物质相互作用方面的研究。目前对DNA与其它小分子物质作用的技术主要有光谱技术、表面分析技术、凝胶电泳、微量热法、电化学技术等。其中电化学技术由于具有灵敏度高、所需药品量少、测试费用低和操作简单等特点而备受人们关注。
DNA修饰电极是用电化学技术研究DNA的一个重要途径，由于制备DNA修饰电极时，DNA的用量很少，并且所制成的DNA电化学传感器体积小、测试费用低、可微型化，使其在DNA研究中的应用范围扩大到传染病、流行病、肿瘤及遗传疾病等的临床检测、食品卫生检验、法医诊断、环境监控等方面。
1.1DNA修饰电极的制备
DNA修饰电极是将单链DNA(ssDNA)片段(长度一般为十几到几千个碱基不等)固定在电极表面形成DNA探针[1],这种ssDNA与目标DNA(靶基因)呈碱基序列互补,在适当的温度、离子强度、pH、缓冲溶液等杂交条件下电极表面探针ssDNA与溶液中的靶基因发生特异选择性杂交, 形成双链DNA(dsDNA) 从而导致电极表面结构的变化变化的情况可通过电极表面电活性指示剂所引起电信号(如电压、电流或电导)的变化体现出来进而可用于靶基因的定性定量分析。DNA修饰电极的制备主要分为以下四个步骤：
（1）将ssDNA固定到固体电极（如玻碳电极、金电极、ITO等），形成DNA探针电极。
（2）在合适的杂交条件下，将DNA探针电极放入含有目标ssDNA的溶液中与靶基因杂交，形成dsDNA。
（3）选择合适的电化学指示剂（如道诺霉素等）完成dsDNA的表达。
（4）根据所选电化学指示剂的不同，选择相应的电化学方法完成电化学信号（如电流、电压）的测定。图1为采用杂交指示剂作为信号分子的核酸修饰电能广泛应用于细茵及病毒感染类疾病诊断、基因诊断、药物分析、DNA损伤研究和环境监测等领域。
1.2 DNA固定方法
1.2.1 吸附法
吸附法通常是通过非共价键作用将DNA 直接吸附到电极表面Brett[3]、Zhou[4]、庞代文[5, 6]等先后利用这一方法将单链DNA固定在石墨电极、玻碳电极、金电极和汞电极表面，制成DNA修饰电极。因为DNA分子中的磷酸骨架带负电荷，可以将预处理好的电极浸入含一定浓度DNA的溶液中，对工作电极施加一定正电位，将DNA吸附到工作电极表面。Wang[7~11]、Erdem[12]和Marrazza[1314]等在恒电位吸附法方面做了大量工作。利用DNA带负电荷的磷酸骨架和带正电的固体基体之间的静电作用也可固定DNA。[15]、周家宏[16]等分别利用带正电荷的聚吡咯、阳离子聚合物壳聚糖和聚赖氨酸将ssDNA固定于玻碳电极表面上，提高了DNA在电极上的固定量。采用吸附法在固定DNA前一般不需要对DNA进行衍生化处理，操作简单、便捷，但DNA探针再生较难。
1.2.2 自组装膜法
陆琪[18] 等人采用先进行-SH化合物自组装得到自组装单分子层再在其上共价键合或吸附固定DNA 的方法制备DNA 修饰电极克服了由于-SH修饰的DNA 难以合成，并且需要分离提纯操作繁琐的缺点。Maeda等[]利用DNA 的5’末端的磷酸基与2-羟基乙基二硫化物(HEDS) 的羟基可反应生成磷酸酯键用凝胶柱将反应混合物进行分离得到纯的5’端修饰的dsDNA。再用HS-将修饰过的dsDNA 固定到金电极表面从而得到DNA修饰金电极。自组装膜法制得的DNA修饰电极其表面高度有序、稳定性高，提高了DNA的杂交效率。但采用此法固定DNA要求DNA纯度较高，且分离操作复杂，并会造成一定的非特异性吸附。庞代文[9]研究了不同基团末端自组装固定DNA。结果表明，羟基末端、氨基末端和羧基末端中通过羟基末端自组装固定DNA量最高，达82.8-87.6%。
1.2.3 生物亲和反应固定法
1.2.4 共价键合法
共价键合法是通过在电极表面引入酰胺键、酯键、醚健等基团使DNA与这些活性基团形成共价键从而固定到电极表面上。或者将核酸衍生，使其带上合适的功能团，用双官能团试剂或偶联活化剂使固体基质与核酸末端之间通过化学键结合起来，从而成功固定DNA。如在氧化的玻碳电极表面, 以一种水溶液的乙基-(3-二甲基丙基) 碳二亚胺盐酸和N- 羟基磺基琥珀酰亚胺作为偶联活化剂, 小牛胸腺DNA 和多聚
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