Summary (text)
Abstract (text)
Development of intrapancreatic transplantable model of pancreatic duct adenocarcinoma in Syrian golden hamsters.
Intrapancreatic and subcutaneous (SC) inoculation of cultured pancreatic cancer cells, derived from an induced primary pancreatic cancer in a Syrian hamster, resulted in tumor take in all recipient hamsters. The intrapancreatic allografts grew rapidly, were invasive, and metastasized into the lymph nodes and liver in 2 of 9 cases. In comparison, SC tumors grew relatively slower and formed a large encapsulated mass without invasion and metastases. Histologically, tumors of both sites showed fairly well-differentiated adenocarcinomas of ductal/ductular type resembling the induced primary cancer. Similar to the primary induced pancreatic cancers, tumor cells of both allografts expressed blood-group-related antigens, including A, B, H, Le(b), Le(y), Le(x), and tumor-associated antigen TAG-72. The tumor cells did not express Le(a), CA 19-9, 17-1A, or DU-PAN-2. The expression of these antigens was retained in the metastases and presented the same patterns of reactivity as the allografts. Thus intrapancreatic transplantation provides a rapid model for production of pancreatic cancer with morphologic similarities to human pancreatic cancer.
PMID: 2000935 [Pubmed - MEDLINE]
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丁香园旗下网站Construction of Staphylococcus aureus vaccine EsxA-EsxB-Flic and the purification of fusion protein--《Heilongjiang Animal Science and Veterinary Medicine》2016年07期
Construction of Staphylococcus aureus vaccine EsxA-EsxB-Flic and the purification of fusion protein
LI FPAN WXIAO JLIU HYUAN YLIU HWEI QZHANG XShenzhen Huada Gene Research IInstitute of Banna Mini-pig Inbred Line,Yunnan Agricultural UBGI ARK Biotechnology Co.,L
To prevent Staphylococcus aureus( SA) infection,and reduce the economic losses of animal husbandry,2 133 bp Esx A- Esx B- Flic fusion genes were composed by Flic flagellin and Esx A and Esx B as virulence factors SA. The expression plasmid was amplified in Escherichia coli,and the total protein was extracted after lysing the bacteria,and then the Esx A- Esx B- Flic fusion protein was purified through His-Tag. The results showed that the method was simple and efficient,which could obtain lots of purified proteins. The method could be used for novel subunit vaccine against SA fusion protein.
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(C)2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reservedResearch of Intelligent Cleaning Management System Based on RFID--《Microprocessors》2014年02期
Research of Intelligent Cleaning Management System Based on RFID
MA Guo-JIANG BLI Guang-XUE Xiao-College of Internet of Things Engineering,Hohai UChangzhou Key Laboratory of Sensor Networks and Environmental S
To avoid the invalid work and poor management of the city 's cleaners,this paper puts forward and develops a cleaner 's intelligent management system which is based on radio frequency identification technology and ultrasonic sensor technology. The system can detect garbage automatically by ultrasonic sensor and realize monitoring and management of each garbage,as well as identify the electronics tag of each cleaner and inform the cleaner immediately through the RFID technology and 3G communication technology. It has a significant application value because the system conveniently achieves intelligent detection,timely dispose and supervision for the city environment.
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TP315;TP391.45
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YUAN Teng,LI Qiu-hong,YANG Guo-bin,CHENG Lei(Key Laboratory for Micro/Nano Optoelectronic Devices of Ministry of Education, Hunan University,Changsha 410082,China);[J];Transducer and Microsystem T2012-06
Cui Jun1 Li Qingli2 (1. SINOPEC Pipeline Storage & Transport Company,Nanjing Branch 2. School of Information Science,East China Normal University);[J];Electronic T2009-11
XIN Zheng-hua1,LU Hong-mei1,HU Liang-yi2,ZHANG Jia-xi1(1.School of Information Engineering,Suzhou University,Suzhou 234000,C 2.School of Economic and Management,Suzhou University,Suzhou 234000,China);[J];Electronic Instrumentation C2011-06
LIU Bing-kai1, HE Qi-chang1, FAN Xiu-min1, ZHU Ming-hua1, LIU Yi2 (1. Computer Integrated Manufacture Institute, Shanghai Jiaotong University, Shanghai . Shanghai Pudong Solid Waste Administrator Office, Shanghai 200135);[J];Computer E2009-08
WANG Hua-bin1,LUO Zhong-liang2,ZENG Shao-ning1,WENG Shang-di2,HU Jian2(1.Department of Computer Science,Huizhou University,Huizhou 516007,C 2.Department Electronic of Science,Huizhou University,Huizhou 516007,China);[J];Computing Technology and A2012-01
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LI Jin1,WANG Hua2(1.Faculty of Science,Chenggong Campus of Kunming University of Science and Technology,Kunming 650500,C 2.Faculty of Graduate,Chenggong Campus of Kunming University of Science and Technology,Kunming 650500,China);[J];Computer Integrated Manufacturing S2011-12
LI Jin1,WANG Hua2(1.Faculty of Science,Chenggong Campus of Kunming University of Science and Technology,Kunming .Faculty of Graduate,Chenggong Campus of Kunming University of Science and Technology,Kunming 650500,China);[J];Computer Integrated Manufacturing S2012-03
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YANG Yan-SU Hong-ZHOU Yong-XU BHUANG Yan-Yunnan Province Computing Center,Digital Media Technology Key Laboratory of Universities and Colleges in Yunnan Province,Yunnan UComputing Center,Kunming University of Science and TKunming Huahang Electric Company L;[J];Journal of Yunnan University(Natural Sciences Edition);2013-S2
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Wang Ming, Ni Ping, Wang Dong, Li Daode (School of Electrical Engineering and Information, Sichuan University, Chengdu 610064, China);[J];Application of Electronic T2011-01
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(C)2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reservedGeochronology,geochemical characteristics and Hf isotopic compositions of granite in the Hutouya deposit,Qimantag,East Kunlun--《Geology in China》2015年03期
Geochronology,geochemical characteristics and Hf isotopic compositions of granite in the Hutouya deposit,Qimantag,East Kunlun
LI KGAO Yong-QIANHE Shu-LIU Yong-ZHANG Zhao-ZHANG Jiang-WANG Ya-MLR Key Laboratory of Genesis and Exploration of Magmatic Ore Deposits,Xi'an Center of China Geological SNo.3 Geological Prospecting Institute of Q
The Hutouya Pb- Zn polymetallic ore deposit is located in Qimantag, East Kunlun. Intermediate- acid intrusive rocks occur widely in this area, and are closely related to iron polymetallic mineralization. The results of LA- ICP- MS zircon U- Pb isotope dating reval that the granodiorite in the periphery of No. Ⅴore belt was formed at(224.3±0.6) Ma and the orthoclase granitein No. Ⅷ ore belt was formed at(239.7±0.8) Ma. Petrogeochemistry shows that the granodiorite is rich in K and poor in Na, with no strong negative Eu anomalies(δEu=0.68-1.06), and is enriched in Rb, Th, U, K, but depleted in Nb, P, Ti, thus belonging probably to I- type granite series. The orthoclase granite is characterized by high Si O2, high alkali, low TFe O, Mg O, Ca O, P2O5 and Ti O2,strong negative Eu anomalies(δEu 0.08- 0.26), enrichment of Rb, Th, U, K,and depletion of P, Ti, Ba, Sr, belonging probably to highly fractionated I-type granite series. Both of them were formed at the post-collision stage. Variable Hf isotopic compositions of zircon indicate that it experienced magma mixing, which provided large quantities of ore- forming meterials for large- scale polymetallic mineralization.
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FU Jian-ming~(1,2),
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CHEN Shou-jian1,LI Rong-she1,JI Wen-hua1,WANG Chao1,ZHAO Zhen-ming1,WANG Bing-zhang2,DAI Chuan-gu3,WANG Guo-can4(1.Xi'an Institute of Geology and Mineral Resources,Xi'an 710054,Shaanxi,C2.Qinghai Institute ofGeological Survey,Xining 810012,Qinghai,C3.Guizhou Institute of Geological Survey,Guiyang 550005, Guizhou,C4.School of Earth Sciences,China University of Geosciences,Wuhan 430074,Hubei,China);[J];Journal of Earth Sciences and E2008-03
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Tag-TextRank: A Webpage Keyword Extraction Method Based on Tags
Li Peng, Wang Bin, Shi Zhiwei, Cui Yachao, and Li Hengxun
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