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Standard hematoxylin–eosin (H&E) staining was carried out on the formalin-fixed tumor specimens. The sections were viewed and captured using a Zeiss Zxiophot 2 microscope
with a Sony DXC970 color camera 。Entire tumor sections were reconstructed by tiled field mapping and the total tumor area as well as the area of tumor necrosis was determined using a MCID5.5 image analysis program。 Estimates of tumor angiogenesis were made on frozen sections stained with CD31 using the Chalkley counting method on vessel hot-spots. Briefly, the three most vascular areas (hot-spots) were identified subjectively.
The vessels then were counted at high magnification (×250) by applying a 25 points Chalkley grid eyepiece (McCrone Microscopes & Accessories, Westmont, IL) to each hot-spot area and recording the number of points on Hoechst 33342 and CD31 highlighted microvessels. The Chalkley count for an individual tumor was the mean of ten random fields. All the animals in the studies were pre-coded and tumor sections were counted in a blinded fashion.
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通过倾斜视野对所有肿瘤切片进行图象重建，然后使用MCID5.5图象分析程序区别是总肿瘤区还是肿瘤坏死区。通过冰冻切片血管热点区CD31染色后，使用Chalkley计数法评价肿瘤的血管生成。主观的标识出三个最大血管区（热点区）。然后使用25点Chalkley网格目镜(McCrone Microscopes & Accessories, Westmont, IL)在高倍放大(×250)下计数每个血管热点区的血管数－－－这是我自己的翻译，总觉得不妥，并且最后两句实在翻译不下去了，请高手指点。
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Based on yourown tranlation,let me guess:对福尔马林固定的标本进行标准的HE染色。。。。。， 通过倾斜视野对所有肿瘤切片进行图象重建，然后使用MCID5.5图象分析程序区别是总肿瘤区还是肿瘤坏死区。为了计数肿瘤血管密度，使用Chalkley计数法评价血管热点区 冰冻切片的CD31染色。简单地说，主观的标识出三个主要血管区（热点区）。然后使用25点Chalkley网格目镜(McCrone Microscopes & Accessories, Westmont, IL)在高倍放大(×250)下计数每个血管热点区的血管数并且记录 Hoechst 33342 与 CD31标记的微血管的点数。 对每个肿瘤，取Chalkley计数随机10个视野的平均数。所有动物在研究中均进行事先编码，并对切片采用单盲法计数仅供参考！
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“The vessels then were counted at high magnification (×250) by applying a 25 points Chalkley grid eyepiece (McCrone Microscopes & Accessories, Westmont, IL) to each hot-spot area and recording the number of points on Hoechst 33342 and CD31 highlighted microvessels.”我认为楼主对这一句的翻译有些不妥，该句的骨架应为：“The vessels were counted…… by applying……and recording…… ”，applying和recording应同为by的宾语。这样对翻译整句就容易些了。The Chalkley count for an individual tumor was the mean of ten random fields. ——对一个肿瘤的Chalkley计数即取十个随机视野的平均值。All the animals in the studies were pre-coded and tumor sections were counted in a blinded fashion. 所有实验动物都被事先编号，肿瘤切片的相关计数也是采取的盲法。不妥之处，请楼主和高手指点，谢谢！
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呵呵，没想到在我准备发帖的过程中已有高手先我一步，见笑了。
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Never mind, and very good, I think we have the same opinion.
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多谢！The Chalkley count for an individual tumor was the mean of ten random fields。能否再分析下这句！
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The Chalkley count for an individual tumor was the mean of ten random fields。能否再分析下这句！ 随机10个视野的平均数，作为对每一个肿瘤标本的的Chalkley计数
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