elisa原理检测蛋白中il1b应加入多少样本

人蛋白激酶B(PKB)ELISA检测试剂盒
人蛋白激酶B
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人蛋白激酶B(PKB)ELISA检测试剂盒
数值仅供参考,保证产品质量、价格优惠、实验效果好,同时我司为您提供最新价格、说明书、规格、用途、实验原理等相关操作说明。
产品名称:人蛋白激酶B(PKB)ELISA检测试剂盒
英文名称:&PKB ELISA Kit
产品性状:盒装液体。
运输条件:2-8℃低温运输,用干冰或者冰袋低温运输。
待检样本:体液、血清、血浆、细胞培养上清液、尿液、组织匀浆、心房水标本等等。
产品特点:灵敏性高,高效性,吸附均匀,吸附性好,回收利用率高,是一款可以让您放心选购的ELISA试剂盒产品。
产品名称&英文名称&运输条件
人蛋白激酶B(PKB)ELISA检测试剂盒
&PKB ELISA Kit
人蛋白激酶B(PKB)ELISA检测试剂盒检测范围:
人、绵羊、小鼠、大鼠、猪、兔、山羊、牛、马、猪、其它动物细胞因子、植物细胞因子、骨代谢、细胞凋亡、激素内分泌、活性多肽、肝纤维化、自身抗体、血栓与止血、肿瘤、自身抗体科研Elisa检测试剂盒。
ELISA试剂盒检测类型:双抗体夹心法测抗原、双抗原夹心法测抗体、间接法测抗体、竞争法测抗体、竞争法测抗原、捕获包被法测抗体、ABS-ELISA法。
人蛋白激酶B(PKB)ELISA检测试剂盒的优势:
1、专业的elisa试剂盒生产企业(Elisa检测试剂盒厂家)
2、原装进口抗体----高效、灵敏、特异
3、规范包被操作----吸附均匀,吸附性好,空白值低,孔底透明度高
4、先进的优化方案----重复性高,可靠性强
5、购买本公司的ELISA试剂盒,免费代检测,上海地区免费上门取样
6、技术服务要求:专业,规范,高效
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
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二轻欧三芹醇当归酸酯 Canine 重组狗 IL-1 beta / IL1B 蛋白 IL1B
用于含量测定 Macrocarpal K
二轻欧三芹素;二轻三芹醇 Canine 重组狗 EGF / Epidermal Growth Facto 蛋白 (His 标签) EGF
用于含量测定 Chrysothol
律草同 Streptococci 重组恒河猴 HER3 / ErbB3 蛋白 (Fc 标签) ERBB3
用于含量测定 Calycanthoside
脱氧乌头减 Streptococci 重组恒河猴 HER3 / ErbB3 蛋白 (His 标签) ERBB3
用于含量测定 Paulownin
二轻杨梅素 Ferret 重组雪貂 TNF-alpha / TNFA 蛋白 TNF
用于含量测定 3-Hydroxycatalponol
番茄减;番茄苷 Ferret 重组雪貂 CD20 / MS4A-1 蛋白 (His 标签) MS4A1
用于含量测定 Moronic acid
番茄红素;ψ—胡萝卜素;茄红素;西红柿红素;蕃茄红素;番茄紅素 Ferret 重组雪貂 CD4 / Leu-3 蛋白 (His 标签) CD4 502-65-8 用于含量测定 SkiMMin
番泻苷A;蕃泻苷A;番泻甙A Ferret 重组雪貂 CD8A / CD8 alpha chain 蛋白 (His 标签) CD8A 81-27-6 用于含量测定 Teuclatriol
番泻苷B;蕃泻苷B;番泻甙B Canine 重组狗 c-MET / HGFR 蛋白 (His 标签) MET 128-57-4 用于含量测定 Nodosin
番泻苷C;蕃泻苷C;番泻甙C Rhesus 重组恒河猴 HER2 / ErbB2 蛋白 (His 标签) ERBB2
用于含量测定 Butein
番泻苷D;蕃泻苷D Rhesus 重组恒河猴 HER2 / ErbB2 蛋白 (Fc 标签) ERBB2
用于含量测定 Ethyl glucoside
小鼠载脂蛋白H(Apo-H)免疫试剂盒 Mouse apolipoprotein H,Apo-H ELISA Kit
小鼠载脂蛋白E(Apo-E)免疫试剂盒 Mouse apolipoprotein E,Apo-E ELISA Kit
小鼠载脂蛋白B48(apo-B48)免疫试剂盒 Mouse Apolipoprotein B48,apo-B48 ELISA Kit
小鼠载脂蛋白B100(Apo-B100)免疫试剂盒 Mouse apolipoprotein B100,Apo-B100 ELISA Kit
小鼠载脂蛋白B(APOB)免疫试剂盒 Mouse Apolipoprotein B-100,APOB ELISA kit
小鼠载脂蛋白A5(apo-A5)免疫试剂盒 Mouse Apolipoprotein A5,apo-A5 ELISA Kit
小鼠载脂蛋白A1(Apo-A1)免疫试剂盒 Mouse apolipoprotein,Apo-A1 ELISA Kit
小鼠孕激素/孕酮(PROG)免疫试剂盒 Mouse Progesterone,PROG ELISA Kit
小鼠钥孔虫戚血蓝蛋白(KLH)IgM抗体免疫试剂盒 Mouse keyhole limpet hemocyanin(KLH)IgM antibody ELISA Kit
小鼠钥孔人蛋白激酶B(PKB)ELISA检测试剂盒戚血蓝蛋白(KLH)IgG抗体免疫试剂盒 Mouse keyhole limpet hemocyanin(KLH)IgG antibody ELISA Kit
小鼠诱导型一氧化氮合成酶(iNOS)免疫试剂盒 Mouse inducible nitric oxide synthase,iNOS ELISA KIT
小鼠游离脂肪酸(FFA)免疫试剂盒 Mouse free fatty acids,FFA ELISA Kit
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!&&&&&&2.如有必要,请您留下您的详细联系方式!
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小鼠血栓前体蛋白(TpP)酶联免疫试剂盒检测分泌性的成份时,用无菌管收集。离心20分钟左右(转/分)。仔细收集上清。小鼠血栓前体蛋白(TpP)酶联免疫试剂盒检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。点击了解更多产品:小鼠血栓前体蛋白(TpP)酶联免疫试剂盒 Mouse&thrombus&precursor&protein,TpP&ELISA&kit :实验名称, 小鼠血栓前体蛋白(TpP)酶联免疫试剂盒 Mouse&thrombus&precursor&protein,TpP&ELISA&kit :实验别名, 小鼠血栓前体蛋白(TpP)酶联免疫试剂盒 Mouse&thrombus&precursor&protein,TpP&ELISA&kit小鼠血栓前体蛋白(TpP)酶联免疫试剂盒使用&1、在试验之前要将试剂盒在室温下平衡至少&30&分钟。&2、不要重复使用手中的吸头和试管,否则会引起交叉污染,导致试验失败。3、该试剂盒内不包含有传染性试剂。&4、不要混用不同批号的试剂和包被微孔板。&5、试验过程中所有试剂和待测样本应充分混合。&6、包被微孔板应干燥保存,避免受潮。&7、如有剩余试剂请放置&2-8℃保存。&8、试验过程要严格按照说明书操作,先后顺序不得颠倒。&9、如果样本量较大应注意加样时间,避免加样过慢或间隔时间过长。10、使用和贮存底物Ⅱ时应注意避光。&11、试验期间室内温度应始终保持在&18-28℃。并保持室内清洁,无粉尘。12、避免直接接触试剂盒内的液体,一旦接触请尽快用水清洗。&13、在试验过程中不要吸烟、使用化妆品和吃喝。这将对操作者的健康及试验结果造成影响。14、不要用嘴吸取试剂瓶内的试剂。白介素1受体Ⅰ(IL1R1)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Receptor&Type&I&(IL1R1) Rattus&norvegicus&(Rat)白介素1受体Ⅰ(IL1R1)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Receptor&Type&I&(IL1R1) Mus&musculus&(Mouse)白介素1家族成员9(IL1F9)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Family,&Member&9&(IL1F9) Homo&sapiens&(Human)白介素1家族成员9(IL1F9)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Family,&Member&9&(IL1F9) Mus&musculus&(Mouse)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Theta&(IL1F10) Homo&sapiens&(Human)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Eta&(IL1h) Homo&sapiens&(Human)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Zeta&(IL1z) Homo&sapiens&(Human)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Epsilon&(IL1e) Homo&sapiens&(Human)白介素1&(FIL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Delta&(FIL1d) Homo&sapiens&(Human)白介素1&(FIL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Delta&(FIL1d) Mus&musculus&(Mouse)白介素1&(FIL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Delta&(FIL1d) Rattus&norvegicus&(Rat)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Chicken&(Gallus)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Sus&&Porcine&(Pig)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Oryctolagus&cuniculus&(Rabbit)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Homo&sapiens&(Human)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Mus&musculus&(Mouse)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Rattus&norvegicus&(Rat)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Cavia&(Guinea&pig&)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Canis&&Canine&(Dog)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Equus&&Equine&(Horse)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Beta&(IL1b) Bos&&Bovine&(Cattle)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Alpha&(IL1a) Sus&&Porcine&(Pig)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Alpha&(IL1a) Homo&sapiens&(Human)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Alpha&(IL1a) Mus&musculus&(Mouse)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Alpha&(IL1a) Rattus&norvegicus&(Rat)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Alpha&(IL1a) Canis&&Canine&(Dog)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Alpha&(IL1a) Capra&&Caprine&(Goat)小鼠血栓前体蛋白(TpP)酶联免疫试剂盒白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Alpha&(IL1a) Equus&&Equine&(Horse)白介素1&(IL1&)检测试剂盒(酶联免疫吸附试验法) ELISA&Kit&for&Interleukin&1&Alpha&(IL1a) Cavia&(Guinea&pig&)
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该厂商的其他产品
您感兴趣的其他供应欢迎+86-400-890-9989Customer experience is always our first concern. Purchase can be made in your local currency now. Explore our website for more!Choose Language&&&&&Text Size:人 IL-1 beta / IL1B 蛋白&&|&&&&&&作者评价应用种属图像 0">Human&IL1B&蛋白产品信息分子别称:IL-1,IL-1 beta,IL-1B,IL1-BETA,IL1F2蛋白构建:A DNA sequence encoding the mature form of human IL1β (NP_) (Ala 117-Ser 269) was expressed.分子种属:Human表达宿主:E. coli运输说明:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. 0">Human&IL1B&蛋白质控检测纯度:> 95 % as determined by SDS-PAGE生物活性:1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/mL (100μl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL.3. Measured by its binding ability in a functional ELISA.4. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 μg/mL.5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.内毒素:Please contact us for more information.稳定性:Samples are stable for up to twelve months from date of receipt at -70℃预测N端:Met分子量:The recombinant human IL1β consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.缓冲液:Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please
for any concerns or special requirements. 0">Human&IL1B&蛋白使用指导储存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.复溶条件:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 0">Human&IL1B&蛋白SDS-PAGEHuman IL-1 beta / IL1B Protein SDS-PAGEOther IL1B&蛋白&Products 0">10139-HNAE 0">IL1B/IL-1B/IL-1 beta&研究背景 Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc). 0">Human&IL1B/IL-1B/IL-1 beta&参考资料 Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16. Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42. Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004. Product nameProduct nameRelated Products
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北京义翘神州生物技术有限公司保留所有权利 京ICP备欢迎+86-400-890-9989Customer experience is always our first concern. Purchase can be made in your local currency now. Explore our website for more!Choose Language&&&&&Text Size:大鼠 IL-1 beta / IL1B 蛋白 (mature form)&&|&&&&&&作者评价应用种属图像 0">Rat&IL1B&蛋白产品信息分子别称:IL1B蛋白构建:A DNA sequence encoding the mature form of rat IL1B (Q63264-1) (Val 117-Ser 268) was expressed and purified.分子种属:Rat表达宿主:E. coli运输说明:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. 0">Rat&IL1B&蛋白质控检测纯度:> 98 % as determined by SDS-PAGE生物活性:Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED50 for this effect is typically 30-120 pg/mL.内毒素:Please contact us for more information.稳定性:Samples are stable for up to twelve months from date of receipt at -70℃预测N端:Met分子量:The mature form of recombinant rat IL1B comprises 153 amino acids and predicts a molecular mass of 17.4 kDa. The apparent molecular mass of the protein is approximately 35 kDa in SDS-PAGE under reducing conditions.缓冲液:Lyophilized from sterile 40mM Tris, 0.15 M NaCl, pH 7.81. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please
for any concerns or special requirements. 0">Rat&IL1B&蛋白使用指导储存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.复溶条件:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 0">Rat&IL1B&蛋白SDS-PAGERat IL-1 beta / IL1B Protein (mature form) SDS-PAGEIL1B&相关信号通路Other IL1B&蛋白&Products 0">80023-RNAE 0">IL1B/IL-1B/IL-1 beta&研究背景 Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc). 0">Rat&IL1B/IL-1B/IL-1 beta&参考资料 Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16.Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42.Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004. Product nameProduct nameRelated Products
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北京义翘神州生物技术有限公司保留所有权利 京ICP备elisa检测蛋白中il1b应加入多少样本_百度知道
elisa检测蛋白中il1b应加入多少样本
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然后进行梯度设计。样本量问题问的不够清楚哈~推荐结合你的ELISA检测方法,你可以分析一下阳性对照和阴性对照的含量、试剂盒说明书进行问题分析
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